University of Rochester Technical Assoc I (S) in Rochester, New York

Opening

Full Time 40 hours Grade 051 Pediatrics M&D; Pulmonology

Schedule

8:30 AM-5 PM; EVENINGS AND WEEKENDS AS NEEDED; SCHEDULE WILL VARY BASED ON OPERATIONAL NEEDS.

Responsibilities

THIS POSITION HAS AN END DATE OF 7/30/21.

Overview:

The technical associate will serve as laboratory manager, in addition to having primary responsibility for the design, execution, and interpretation of experiments performed in the Division of Pulmonology, Department of Pediatrics. Projects in the lab focus on understanding the mechanisms of airway injury and aberrant repair leading to early lung function decline. The mode of research in the laboratory includes cell culture, cytometry, western blot analysis and serologic methodology. Research in the laboratory is conducted using both in vivo (small animal models) as well as in vitro cell culture models with inhalation exposure systems. The technical associate will be expected to design and conduct experiments, as well as present and interpret experimental results. When possible, the associate will be encouraged to present data at meetings and co-author papers.

The associate must be comfortable working with, and have the ability to handle and euthanize small animals, specifically rats and/or mice. Additional experience in inhalation toxicology and exposure systems is also preferred, but not required.

The associate will supervise the daily operation of the laboratory. The associate will be responsible for several routine, non-technical activities, including writing and optimizing experimental protocols, development and maintenance of lab safety protocols, purchase of laboratory supplies and equipment, monitoring of laboratory inventories, and maintenance of expense records.

The associate will have several supervisory roles, including training and supervising undergraduates, graduate students, and pediatric residents or fellows who rotate through the laboratory. In addition, as laboratory personnel and projects change, the associate will assist in the hiring and training of new technicians, graduate students, and postdocs. The associate will monitor the quality of daily work and will be responsible for ensuring proper laboratory documentation and adherence to lab safety protocols.

Responsibilities:

Independently design and perform experiments

  • Independently designs, performs, and modifies experiments based on analyses of the results. Assist with the development, implementation and execution of new protocols.These include but are not limited to flow cytometry, tissue culture based assays, tissue staining (hematoxylin & eosin, trichrome), western blot analyses, polymerase chain reaction protocols, blood separation and ELISA protocols.
  • Routine maintenance, treatment, euthanasia, dissection, and tissue preparation of rats and/or mice.
  • Closely collaborates with the PI on the design, implementation, and modification of inhalation exposure systems for small animals (rats and/or mice) and in vitro co-culture systems.

Direct and manage day-to-day laboratory operations.

  • Oversees and manages laboratory budget to ensure laboratory stays within budget parameters and forecasts variances based on expenditures. Makes recommendations to modify budget lines to PI. Makes recommendations to PI in annual lab budget planning. Supervise ordering activities of other members of the lab.
  • Train, supervise, direct and assist students, residents, fellows and technicians in the laboratory in terms of execution and troubleshooting of their experimental work including research compliance. Monitor quality of day-to-day work of students, residents and technicians. Assist PI with interviewing and hiring decisions, and with evaluating and resolving performance issues.
  • Provide instruction to other laboratory personnel in general laboratory research techniques. Orients new and collaborating laboratory personnel (primarily post-doctoral fellows, MD researchers and other technicians) to the laboratory-related procedures.
  • Monitors and ensures training and compliance with Environmental Health and Safety – Occupational Safety Unit, Safety Training for Research Laboratory Personnel requirements specific to laboratory for all laboratory personnel. Ensures that health and safety practices are maintained in accordance with University policy and the law. Records laboratory and other training as required.
  • Oversees regulatory issues including biosafety, environmental health and chemical safety. Responsible to implement changes, respond to issues related to but not limited to laboratory inspections conducted by Fire Marshall and Environmental Health and Safety.
  • Identify and troubleshoot laboratory and personnel issues, propose resolutions and implement improvements.Monitor outcomes to ensure issues are resolved and improvements sustained.

Data Interpretation and Analyzes

  • Analyze and interpret data acquired during experiments and literature reviews, and disseminates to PI and/or weekly laboratory meetings internal and external to Department, and makes recommendations as to lab direction based on current findings and literature.

Maintains Lab Supplies and Equipment

  • Oversees maintenance, management, and/or repair of equipment; coordinates ordering of supplies and equipment and trains people in the appropriate use of equipment. Has the ability to make decisions on purchases of supplies and all equipment. Will perform in depth comparative research to obtain the best product at the best price and provide informed recommendations to the PI for decision making. Meets with vendors to review equipment/laboratory needs.
  • Maintains organized ordering records and tracks missing deliveries; troubleshoots and resolves supply and/or equipment errors.

Specimen Shipping and Organization

  • Responsible for routine freezer organization and shipping specimens to and receiving specimen from collaborators and funding agencies per protocol. Responsibilities include training personnel in databases, tracking of study samples and freezer organization procedures. Complete and monitor shipping certifications for personnel.

Contributes Writing of Protocols, Papers, and Presentations

  • Independently writes and edits sections of manuscripts dealing with methodology for publication, posters and presentations for dissemination at local, regional and national meetings.
  • Develop, write and modify standardized operating procedures based on results of complex experiments.
  • Establish and troubleshoot new laboratory protocols evaluating effectiveness and modifying based on analyses.

Requirements:

Bachelor’s degree with major course work in field of assignment, and one year of related work experience involving direct assistance to researchers; or any equivalent combination of experience, training and education. Prefer experience in Pulmonary Medicine. Prefer experience in discipline with 2 to 4 years specialized experience in related field, or equivalent combination of education and experience. Knowledge of basic pulmonary physiology, molecular biology, tissue culture method, and animal handling experience is preferred. Specific skills necessary for this position include knowledge of general laboratory safety processes, universal precautions, flow cytometry, aseptic cell culture technique, PCR and qPCR, ELISA, and animal maintenance/treatment/handling/tissue preparation. Familiarity with general laboratory equipment including biosafety cabinets, centrifuges, freezers & cold room, pH meter, autoclave, PCR machines, pipettors, fume hoods, spectrophotometers & ice machine, balances, microscopes, incubators, power supply & gel boxes is expected.

Additional skills may be required and training provided as needed. Thorough documentation of results in written and electronic database formats will be required. Requires Advanced computer skills including MS Word and Excel. Experience using flow cytometry software, statistical software packages such as Prism are preferred. Skills in basic database management are preferred.

Applicants are not expected to have mastery of all of these skills and will receive further training in the position.

Demonstrates skill in communication with the Principal Investigators and other members of the laboratory team including communicating effectively with laboratories internal and external to the department. Demonstrates skill in prioritizing assignments and is able to effectively multitask. Proactive in resolving problems and implementing quality improvement. Demonstrates competence in the technical skills as detailed by the job description. Demonstrates skill in supervising students and lab technicians. Demonstrates high level of attention to detail. Must be able to work independently and without direction in the day-to-day operations of the laboratory. Must be able to maintain detailed records. The Technical Associate will meet with Principal Investigators once weekly for 1-2 hours to discuss results of experiments, present plans for experiments, make recommendations about new experiments, and discuss lab management issues.

EOE Minorities/Females/Protected Veterans/Disabled

Job Title: Technical Assoc I (S)

Location: School of Medicine & Dentistry

Job ID: 208822

Regular/Temporary: Regular

Full/Part Time: Full-Time