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University of Rochester Technical Assoc I (S) - 215033 in Rochester, New York

Technical Assoc I (S) Job ID 215033

Location School of Medicine & Dentistry Full/Part Time Full-Time Favorite Job Regular/Temporary Regular Opening

Full Time 40 hours Grade 077 OBGYN Research

Responsibilities

Position Summary:

Under the supervision of Dr. Falsetta, the position is full-time in a laboratory studying the vulvar pain condition known as localized provoked vulvodynia. There is also a minor clinical coordinating component (5%) that involves working with clinical faculty and staff to identify patients eligible for the study, obtain patient consent, and pick up patient tissue specimens for laboratory analysis. Previous clinical coordinator experience is not required.

The position requires daily utilization of laboratory skills, including but not limited to cell line and primary cell culture, aseptic technique, microbiological culture, microscopy, nucleic acid isolation (RNA and DNA), standard and quantitative PCR, siRNA knockdown, protein overexpression, flow cytometry, genetic/cloning techniques, protein quantification, Western blot analysis, ELISAs, EIAs, Luminex, primary cell isolation and purification (from human and mouse tissues), the handling of mice, and procedures on mice (subcutaneous injection, anesthesia, treatment applications, pain testing, post-mortem tissue collection, gavage, etc.).

The position requires a high level of independence with only occasional suggestions in modifying, varying, or adapting laboratory procedures to meet the special needs of the research. A qualified applicant will be adept at troubleshooting and adapting protocols and techniques as needed. The position requires the ability to be proactive and identify problems before or when they arise and be able to triage them.

Upon the completion of experiments, the data must be processed, interpreted, and organized in a brief written report with presentations to be made at lab meetings on a regular basis. Furthermore, the technician will assist with the preparation of charts, graphs, and tables for publications, presentations, and grant applications. There may be the requirement to write short sections of text covering methodology or review text pertaining to procedures performed by the technician. Regularly scheduled progress meetings will be held with Dr. Falsetta. An understanding of the experiments and relevant up-to-date knowledge of the projects’ background is expected, which may include the need to perform literature searches and read primary research articles and reviews.

Duties also include serving as the laboratory safety correspondent, ordering and maintaining supplies, and maintaining laboratory organization, data safety sheets, chemical inventories, and safety protocols.

Responsibilities:

  • Bench/laboratory work: formulate experimental procedures and use laboratory techniques to process the experiments in the appropriate manner.

  • In vivo mouse model

  • Immunological assays: EIA, ELISA, Luminex, Flow

  • Protein extract preparation and Western Blot analysis

  • Nucleic Acid Isolation and RT-PCR/PCR

  • Cell/Tissue Culture

  • Data processing and figure formatting: after compiling data from experiments, the data must be analyzed. Then, it must be organized to publication quality and presented.

  • Administrative/laboratory organization: serving as the laboratory safety correspondent, ordering and maintaining laboratory stocks and supplies, and maintaining laboratory organization, writing/modifying laboratory protocols, and maintaining data safety sheets and safety protocols.

  • Reporting: regularly meeting with Dr. Falsetta to discuss the current state of the project. Attending and actively participating in laboratory meetings to present analyzed data.

  • Clinical coordinator duties: identifying patients eligible for the study by reviewing the surgical calendar and then coordinating with the clinical faculty and staff to consent patients and pick up tissues and samples when ready.

    Qualifications:

    Bachelor’s degree in biological sciences, with an emphasis on microbiology, immunology, and/or molecular and cellular biology. Experience in the techniques outlined above and other technical or research experience is highly valuable. The successful applicant will possess great attention to detail, as well as knowledge of Excel and Word, and be proficient with routine laboratory calculations.

    How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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