University of Rochester Laboratory Technician IV in Rochester, New York
Full Time 40 hours Grade 079 Environmental Medicine
8:30 AM-5 PM; OCC WKENDS
The laboratory’s research focuses on understanding the mechanisms and treatments of chronic obstructive pulmonary disease/emphysema, asthma, and pulmonary fibrosis. Performs research laboratory assignments involving some variety in accordance with standard practices and procedures. The candidate will be responsible for maintaining the colonies of transgenic and knockout mouse, breeding of mice, record-keeping, animal protocols submissions/modifications, working closely with Vivarium and DLAM staff, cigarette smoke inhalation exposure to mice, carrying out the laboratory experiments in vitro cell culture and in vivo (cigarette smoke, e-cigarette and waterpipe/hookah exposure to mice and procedures) and assisting several technicians and fellows in the laboratory, in addition to managing all aspects of animal protocols and laboratory work from data generation to data analysis. Responsibilities include placing orders for animals and lab supplies, scheduled maintenance for lab instruments, oversight and routine biochemical and molecular biological assays. Responsibilities will also include: engaging in experimental design and independent execution of above methods/protocols/assays; overseeing general laboratory functions such as inventory and ordering of supplies; and training of new laboratory personnel in fundamental lab techniques. Work with students and fellows/coordinators on basic and clinical research projects in collecting biological samples and perform experiments and assays (cell biology, biochemical and molecular biology techniques) in cells and biological tissues. Execution of experiments will require proficiency in cell and molecular biology techniques including, but not limited to: RNA isolation, quantitative PCR, DNA cloning and expression, as well as cell culture, SDS-PAGE, Western blotting, immunocytochemistry and immunohistochemistry. Plans and conducts standardized technical procedures and lab experiments. Keep track of Institutional Biosafety Committee approvals, EH&S;, IRB/RSRB and DLAM training and annual updates for laboratory personnel and compliance. Co-ordination of clinical studies with clinical research center and the lab, assist in subjects recruitment and collection of biological samples. Weekend work will be required at times, based upon experimental schedules. Interest in and willingness to learn new techniques are essential job components. This position is ideal for a motivated and independent research personnel who is keen to learn new techniques/research and involve in scientific publications.
Techniques to be performed: Western blotting, immunostaining, lung function measurements experiments, ELISA, Northern blotting, ribonuclease protection assays, tissue section staining, Diff-Quick staining, immunohistochemistry, in situ hybridization. RNA isolation and DNA isolation, ChIP assay, EMSA (gel shift assay), RT-PCR, restriction enzyme digests, plasmid purification and extraction, analyzing results and record keeping. Perform new experiments and assays in cells and tissues. Assist undergraduate students, graduate students and postdoctoral fellows to learn and perform assays, routine techniques/methods, and procedures for their experiments.
Duties include maintaining the colonies of transgenic and knockout mice; breeding of mice, record-keeping, animal protocols submissions/modifications, working closely with Vivarium and DLAM staffs, cigarette smoke inhalation exposure to mice and carrying out the laboratory experiments in vitro and in vivo (cigarette smoke exposure to mice) exposing cigarette smoke to mice, preparing cigarette smoke extracts. Animal Care: animal cigarette smoke exposures and inhalation (oral, s.c., i.m., i.v., or i.p.), lung and heart removal, dissection and tissue preparation. Maintaining UCAR paperwork/protocols, ordering new rodents, check animals daily, oversight of DLAM for their maintenance of one animal room. Keep track of various training and annual updates for all laboratory personnel so that the labs are in compliance with these offices. Ensure all other laboratory personnel are up-to-date on required laboratory and animal usage training. Co-ordinating of clinical studies with clinical research center and the lab, assist in subjects recruitment and collection of biological samples. Pick up samples from Clinical Research Labs and process for analyses.
Orders equipment and laboratory supplies, sets up experiments, cleans and performs routine maintenance on equipment. Duties will include filing of order forms, keeping schedule for equipment maintenance, maintenance of computer records for lab and equipment, maintenance of chemical inventory, autoclave items, interact with sales representatives, some cleaning and storing of glassware, oversight and record keeping for hazardous waste disposal. Plans and conducts standardized technical procedures and lab experiments. Set-up and operates equipment or instrumentation. Oversee and maintain laboratory.
Maintain laboratory supplies and inventories (chemicals, animals and equipment). Serve in a managerial role to maintain laboratory inventory and address needs for instrument repair.
Collaborate with other personnel within the laboratory and investigators from other laboratories.
Participate in lab meetings and seminars. Assist the Principal Investigator with preparing figures, data sets and written text for grants and publications.
Associate’s degree in biological sciences plus 4-5 years’ experience is required; Bachelor’s/Master’s degree in biological or Life Sciences (preferred), plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. Experience in clinical research coordination (human subjects), animal (mouse) work and cell culture work with handling multi-tasks. Experience in routine biochemical and molecular biological techniques, and practical laboratory and animal experience. Must be willing to work with animal tissues. Ability to communicate clearly both verbally and in writing is essential. An ability to work independently and as a member of a team is also required. Good organizational skills, ability to use common sense, and demonstrate attention to detail are necessary. Must be able to work some evenings and weekends to conduct experiments. Serve as liaison of laboratory to Departmental and University administration. Assist in administrative aspects for submission of publications, grant proposals, and progress reports.
EOE Minorities/Females/Protected Veterans/Disabled
Job Title: Laboratory Technician IV
Location: School of Medicine & Dentistry
Job ID: 209248
Full/Part Time: Full-Time