University of Rochester Laboratory Technician IV (S) in Rochester, New York
Full Time 40 hours Grade 079 Ped Allergy/Immunology-SMD
8:30 AM-5 PM; OCC EVENINGS; RARE WKENDS/HOL; SCHEDULE MAY VARY BASED ON OPERATIONAL NEEDS
The position will provide technical support for experiments performed in an Allergy and Immunology Laboratory. Projects in the lab focus on understanding factors predisposing to atopic diseases with an overall goal of food allergy prevention. The Technician will work independently, organize and troubleshoot experiments, and integrate different experimental approaches to address scientific aims. The Technician must be able to work in a collaborative lab environment internal and external to the Division and Department.
The Technician will plan and perform routine molecular and immunobiology techniques as well as technical functions including the development and maintenance of laboratory safety protocols, experimental protocols, purchase and maintenance of laboratory supplies, equipment and budgeting. The Technician will oversee and train new laboratory personnel including undergraduate and graduate students in routine research techniques. Working with mouse models may become necessary in the future. Results from experiments performed by the Technician will be used both in publications and grant proposals.
Isolate blood cell fractions using density centrifugation, analyze cells by flow cytometry and immuno-cytochemistry, analyze human serum and milk using ELISA and Luminex multiplex immunoassays.
Molecular Biology Techniques
Develop, implement, and troubleshoot experiments utilizing molecular biology techniques, including isolation of RNA and DNA, PCR, RTPCR, and qPCR.
Cell Biology Techniques
Culture immortalized cell lines and isolated human blood cells using basic cell culture techniques.
Analyze data, provide written summaries of data for grants and publications; develop figures for grants and publications. Use software applications to generate test reports; present results including results of literature reviews to supervisor and PI and at lab meetings with the research team. Prepare figures and tables for grant applications or to present data. Critically appraises research methodology and identifies issues or potential for issues and makes recommendations to PI and Supervisor for resolutions. Prepares basic statistical testing including graph generation. Assists with budget preparation.
Prepare general buffers and media. Oversee maintenance, management, and/or repair of equipment. Independent management of the laboratory's chemical inventory. Monitor and coordinates ordering of lab inventories ensuring orders for equipment and supplies as properly processed. Train individuals in the appropriate use of equipment. Has the ability to make decisions on purchases of supplies and all equipment. Will perform in depth comparative research to obtain the best product at the best price and provide informed recommendations to the PI and Supervisor for decision making. Meets with vendors to review equipment/laboratory needs. Responsible for orderly appearance of the laboratory.
Train, supervise new lab personnel and students; direct and assist laboratory personnel, undergraduate and graduate students and technicians in the laboratory in terms of execution and troubleshooting of their experimental work including research compliance around animal care and routine research techniques. Monitor quality of day-to-day work of these individuals. With supervisor, involved in interviewing and hiring decisions; evaluating performance and resolving performance issues.
- Provide instruction to other laboratory personnel in BSL2 procedures and general laboratory research techniques. Orients new and collaborating laboratory personnel to laboratory-related procedures.
Monitors and ensures training and compliance with Environmental Health and Safety – Occupational Safety Unit, Safety Training for Research Laboratory Personnel requirements specific to laboratory for all laboratory personnel. Ensures that health and safety practices are maintained in accordance with University policy and the law. Records laboratory and other training as required. Establish and troubleshoot new laboratory protocols evaluating effectiveness and modifying based on analyses. Contribute to and manage RSRB, UCAR and IBC protocols.
- Oversees regulatory issues including biosafety, environmental health and radiation usage.Responsible to implement changes, respond to issues related to but not limited to laboratory inspections conducted by Fire Marshal and Environmental Health and Safety.
Attends weekly laboratory meetings and relevant seminars pertinent to the research goals of the laboratory. Meets with PI on a regular basis to discuss research findings and make recommendations regarding future directions.
AAS degree in appropriate discipline plus 4-5 years specialized experience in related field; or equivalent combination of education and experience.
Prefer BS in related field of study with 2-3 years’ experience preferred. Experience working with tissue culture and flow cytometry required. Candidate must have excellent command of oral and written English language and able to work in a collaborative work environment. Attention to detail and meticulous ability to maintain records is essential.
Advanced computer skills including MS Word and Excel, required. Experience using flow cytometry software, statistical software packages such as Prism are preferred.
Occasional evening or weekend hours may be necessary. Schedule varies based on operational needs.
Knowledge of general laboratory safety processes, universal precautions, aseptic cell culture technique, handling of mice and practice of rodent euthanasia required. Thorough documentation of results in written and electronic database formats will be required. Skills in basic database management and generation of publication-quality figures preferred. Needs to be proficient in anesthetic techniques.
EOE Minorities/Females/Protected Veterans/Disabled
Job Title: Laboratory Technician IV (S)
Location: School of Medicine & Dentistry
Job ID: 206490
Full/Part Time: Full-Time