University of Rochester Laboratory Technician III (S) in Rochester, New York


Full Time 40 hours Grade 077 Medicine M&D-Nephrology; Div


8 AM-4:30 PM


Under general supervision and with some latitude for exercising independent technical judgment, is responsible for carrying out research procedures related to tissue culture, isolation of primary human and mouse B cells, mouse vaccination, ELISA, ELISPOT, and western blotting. Also responsible for performing molecular techniques including protein purification, DNA cloning, DNA and RNA extraction, insect cell culture, and performing Luminex assays testing human and rodent sera. Additional work with rodent colonies, including vaccination, intransal influenza infection, blood harvesting, euthanasia, organ dissection and primary B and T cell isolation and culture. Ability to perform and co-ordinate multiple tasks on a daily basis. Will ensure maintenance of laboratory supplies, equipment and proper disposal of all materials.

Specific Duties:

  • Plans and performs procedures in cell and organ culture of bone. Performs protein immunoassays, realtime QPCR and chemical analyses and bioassays and research experiments involving bone tissue.
  • Collects and analyzes scientific data using computerized software, adapting programs to meet the needs of individual experiments. Maintains technical expertise and awareness of trends in renal physiology research through professional journals and participation in research team meetings.
  • Operates and maintains complex experimental testing equipment such as a blood-gas machine, Ca ion analyzer, spectrophotometer, pH meters, CO2 incubator, and the like.
  • Maintains inventory control of all laboratory supplies needed for research activities and ensures proper disposal of all laboratory materials.
  • Prepares charts, graphs, and tables and assists in the preparation of materials for publication.
  • Assists in daily maintenance of experimental rodent colonies for research; coordinates special surgical procedures with Vivarium staff. Performs specialized surgical procedures in rodents.
  • Performs literature searches for material relevant to research projects.


Minimum of an Associate’s degree in appropriate discipline plus 2-3 years specialized experience in related field: or equivalent combination of education and experience. Prefer Bachelor's degree in science.

Prefer at least 2-3 years related experience, involving direct assistance to researchers but not essential. Experience with tissue culture desirable. Computer skills with Excel and Adobe graphics software, and/or bioinformatics experience also desirable.

EOE Minorities/Females/Protected Veterans/Disabled

Job Title: Laboratory Technician III (S)

Location: School of Medicine & Dentistry

Job ID: 209337

Regular/Temporary: Regular

Full/Part Time: Full-Time