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University of Rochester Laboratory Technician III (S) - 224067 in Rochester, New York

Laboratory Technician III (S)

Job ID

224067

Location

School of Medicine & Dentistry

Full/Part Time

Full-Time

Favorite Job

Regular/Temporary

Regular

Opening

Full Time 40 hours Grade 077 Ctr Musculoskeletal Research

Schedule

8 AM-4:30 PM

Responsibilities

Position Summary:

This is a Technical position within Dr. Jennifer Jonason’s Laboratory in the Center for Musculoskeletal Research consisting of laboratory work using genetic mouse models, human tissue samples, and cell culture systems to better understand skeletal development and disease, in particular, osteoarthritis. This position requires mouse handling, mouse recovery surgery, tissue harvest and sample processing for histology and micro-CT analyses, molecular biology skills (PCR, gel electrophoresis, Western blotting, flow cytometry, etc.), cell culture skills, experience in data analysis/interpretation, excellent organizational skills, and careful attention to detail.

Specific Responsibilities:

Breed, maintain, and genotype mice for multiple projects within the lab and keep detailed electronic inventory records of the mouse colonies.

Perform injections and minor recovery surgeries (intra-articular injections and meniscal/ligamentous injury) on mice for the support of ongoing research projects.

Dissection of embryonic and postnatal mouse skeletal tissues for histology, micro-CT, or RNA isolation, embedding and sectioning of paraffin or frozen tissues, histological staining, histomorphometry, and immunohistochemistry of samples for ongoing research projects.

Procurement of human tissues from Surgical Pathology and Orthopaedics clinical coordinators for culture, histology, micro-CT, or RNA isolation.

Isolation and culture of primary skeletal cells and cell lines (chondrocytes, synoviocytes, and mesenchymal progenitor cells). Isolation and analysis of RNA and protein from cell cultures for real-time quantitative PCR, Western blotting, reporter assays, and flow cytometry for ongoing research projects.

Maintenance of a detailed lab notebook/electronic records with experimental detail, methods, data compilation, analysis, and interpretation included.

Assist with general lab maintenance (defrost freezers, stock supplies, place orders, etc.) when necessary.

Requirements:

Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. More than one-year previous experience with mouse work and cell culture is preferred.

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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