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University of Rochester Laboratory Technician III (S) - 223667 in Rochester, New York

Laboratory Technician III (S)

Job ID

223667

Location

School of Medicine & Dentistry

Full/Part Time

Full-Time

Favorite Job

Regular/Temporary

Regular

Opening

Full Time 40 hours Grade 077 Urology M&D

Schedule

8 AM-5 PM; OCC WKENDS/HOL

Responsibilities

Position Summary:

The position will provide technical support for experiments performed in Dr. Yi-Fen Lee’s Laboratory. Projects in the lab focus on tumor derived extracellular vesicles (TEVs), often called exosomes, for their roles in cancer recurrence and metastasis with a focus on urological cancers such as bladder and prostate cancers. The lab also investigates the impacts from enviromental factors, especially smoking and E-liquids, on the TEV-mediated oncogenic pathways in experimental models. The individual is expected to work independently, organize and troubleshoot experiments, integrate different experimental approaches to address scientific aims, and be able to work in a collaborative lab environment internal and external to the Department of Urology.

The individual will plan and perform routine cellular and molecular biology experiments as well as extracellular vesicle charaterization and will assist new laboratory personnel including residents, undergraduate and graduate students in routine research techniques. Working with human cell culture and mouse models will be required. Results from experiments performed by the individual will be used both in publications and grant proposals.

Responsibilities:

Extracellular vesicle related techniques

Collection and characterization of extracellular vesicles ioslated from cancer cells under various environmental stresses as well as from patients’ urine and blood using gradient centrifugation, nanoparticle tracking analysis of cells, and immuno-cytochemistry.

Molecular biology techniques

Develop, implement, and troubleshoot experiments utilizing molecular biology techniques, including siRNA targeted knocking down genes of interest, isolation of RNA and DNA, PCR, RT_PCR, and qPCR,

Cell biology techniques

Culture various cell lines using basic cell culture techniques and characterize cancer cell behavior, such as proliferation, migration, invasion and adhesion..

Analyze data, provide written summaries of data for grants and publications; develop figures for grants and publications. Use software applications to generate test reports; present results including results of literature reviews to supervisor and PI and at lab meetings with the research team. Prepare figures and tables for grant applications or to present data. Critically appraises research methodology and identifies issues or potential for issues and makes recommendations to PI and Supervisor for resolutions. Prepares basic statistical testing including graph generation. Assists with budget preparation.

Asssist new lab personnel residents, undergraduate and graduate students and technicians in the laboratory in terms of execution and troubleshooting of their experimental work including research compliance around animal care and routine research techniques. Monitor quality of day-to-day work of these individuals.

Attends weekly laboratory meeting, Journal Club, and relevant seminars pertinent to the research goals of the laboratory. Meets with PI on a regular basis to discuss research findings and make recommendations regarding future directions.

Requirements:

Associates degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience.

Experience working with tissue culture and flow cytometry required. Candidate must have excellent command of oral and written English language and the ability to work in a collaborative environment. Attention to detail and ability to maintain meticulous records is essential.

Advanced computer skills, including MS Word and Excel, required. Experience using Photoshop software, statistical software packages such as Prism are preferred.

Occasional evening or weekend hours may be necessary. Schedule varies based on operational needs.

Knowledge of general laboratory safety processes, universal precautions, aseptic cell culture technique, handling of mice and practice of rodent euthanasia required. Thorough documentation of results in written and electronic database formats will be required. Skills in basic database management and generation of publication-quality figures preferred. Needs to be proficient in anesthetic techniques.

Preferred qualifications:

Bachelor’s degree in related field.

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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