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University of Rochester Laboratory Technician III - 236677 in Rochester, New York

Laboratory Technician III Job ID 236677

Location School of Medicine & Dentistry Full/Part Time Full-Time Favorite Job Regular/Temporary Regular Opening

Full Time 40 hours Grade 077 Pharmacology & Physiology

Schedule

8:30AM-5:00PM

Responsibilities

General Purpose (see percentages below)

Responsible for the maintenance, breeding, genotyping, and organizing multiple transgenic mouse colonies. Also responsible for overall functioning of the laboratory including ordering supplies, making stock solutions, equipment maintenance, and training. Willingness to work with mice is required and previous experience is preferred. Ability to work in a lab team environment with a diverse group of personnel is essential. Excellent opportunity to gain experience and training in a research lab contributing to ongoing scientific projects and publications.

Typical Assignments and Responsibilities (see percentages below)In accordance with clearly-outlined objectives, requirements and approaches, and with supervision, will conduct technical procedures that are routine and non-routine in nature. Will organize and manage mouse colonies. Will be responsible for ordering laboratory supplies and interacting with vendors. Will work with computers, scientific software, Excel, Word, etc. With latitude for modifying methods and techniques, will analyze data, prepare reports, present data at laboratory meetings, and make recommendations.

Specific Responsibilities:

30% General laboratory maintenance. Order supplies and work closely with departmental administrative office staff on purchasing and account issues. Prepare common reagents such as cDNA and provide support and technical assistance on experimental problems that may arise during the course of their experiments. Maintain functionality of lab equipment, as well as the overall organization of the lab, and comply with all institutional laboratory safety procedures. Maintain careful laboratory notebooks, analyze data on PCs, and design new protocols.

25% Breeding of mouse colonies. Maintenance and breeding of multiple lines of transgenic mice. Responsibilities include generation of ear punches and tail snips, maintaining breeding records and setting up weekly breeding schedules.

25% Mouse genotyping. Responsibilities include PCR analysis of genomic DNA obtained from ear punches and tail snips, as well as detailed record-keeping of mouse genotypes in laboratory database.

15% Preparation/maintenance of cell cultures. Following training, will generate and maintain cell cultures (e.g. HEK293 cells, primary neuronal cultures, etc.). Prepare culture media and solutions, feed cells regularly, propagate and freeze cells for later use.

5% Research Analysis, Presentation, Searches. Prepare literature searches as needed. Analyze and present findings to PI and/or in laboratory meetings.

Other job duties as assigned.

Position Requirements

  • Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; B.A. or B.S. degree in Biology, Biochemistry, Neuroscience or other appropriate discipline strongly preferred; or equivalent combination of education and experience. Willingness and ability to work with mice is required.

  • Strong computer skills, including experience with scientific software, Excel, Word, etc.

  • Ability to work in a lab team environment with a diverse group of personnel is essential.

  • Ability to work with minimal supervision once trained and to listen to, convey, and exchange information with other laboratory personnel.

  • Requires ability to perform experiments with precision and the ability to perform mathematical calculations using algebra and practically apply percentages, fractions, and ratios.

  • Ability to communicate effectively, including written communication; follow instructions; and record information accurately.

  • Must have excellent interpersonal skills and strong attention to detail.

  • This position may entail prolonged periods of standing or sitting and requires the safe use of toxic chemicals.

  • License/Certification Required: Institutional certification for laboratory personnel (e.g., laboratory safety training, vivarium, etc.) will be required after the start of employment.

The University of Rochester is committed to fostering, cultivating, and preserving a culture of equity, diversity, and inclusion to advance the University’s mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion/creed, sex, sexual orientation, citizenship status, or any other status protected by law. This commitment extends to the administration of our policies, admissions, employment, access, and recruitment of candidates from underrepresented populations, veterans, and persons with disabilities consistent with these values and government contractor Affirmative Action obligations.

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