University of Rochester Laboratory Technician III - 223474 in Rochester, New York
Laboratory Technician III
School of Medicine & Dentistry
Full Time 40 hours Grade 077 Pharmacology & Physiology
Responsible for carrying out experiments related to 3-D rendering of anatomical pathways and circuits in the brain, specifically the cortical and basal ganglia system. This requires performance of multiple techniques including scanning microscope slides, adjusting images, and analyzing data using complex software programs. Other tasks may include some laboratory skills such as, preparing microscope slides, and mounting tissue sections onto slides. Knowledge of basic biology and computer skills are required. Willingness to work with animal tissue and the ability to work in a laboratory team environment with a diverse group of personnel is essential.
Typical Assignments and Responsibilities:
In accordance with broadly outlined objectives, requirements and approaches, plans and conducts technical procedures, non-routine and complex in nature. With latitude for modifying methods and techniques, records, computers, and analyzes data, prepares reports, presents data at laboratory meetings, and makes recommendations. Has working knowledge of computers, scientific software, IMOD, Photoshop, Illustrator, etc.
Prepare sections for each model. Work in an independent manner with minimal supervision. Download MRI images from the MRI facility and convert each image into a separate tiff file. Prepare histological sections to be imaged and convert each image into a separate tiff file. Align histological images with MRI images based on anatomical landmarks. Import information into large databases. May require occasional wet lab bench work. This includes mounting sections onto slides and cover slipping microscope slides.
Development of maps of neuronal cell locations. Perform analysis using a combination of software programs to determine the locations of labeled cells in the cortex. Use Excel graph results and prepare documents to be given to the PI. Make recommendations for additional analyses, and modify as needed. Analyze and to present findings to PI and/or in laboratory meetings.
Develop models from new experiments. Work in an independent manner with minimal supervision. Design, plan models from new experiments to determine the organization of specific pathways and terminal fields from different cortical areas. Use IMOD to import new data into the atlas models. Use Photoshop to create images and to prepare documents to be given to the PI. Make recommendations for future experiments, and modify experiments as needed. Analyze and present findings to PI and/or in laboratory meetings.
Develop 3-D brain atlas models. Work in an independent manner with minimal supervision. Design, plan and develop the atlas models that are developed to incorporate new data. This involves selecting, determining the correct orientation for each section, and building the model. Requires operation of complex computer programs, including Photoshop, IMOD, and illustrator. Make recommendations for future atlas models, and modify models as needed. Analyze and to present findings to PI and/or in laboratory meetings.
General database maintenance. Provide support and technical assistance on experimental problems that may arise during the course of their experiments. Order supplies related to computer analysis. Maintain functionality of lab databases, including injection sites, and references. Perform literature searches, assist with grant and manuscript preparation, and make scientific contributions to research projects. Other miscellaneous laboratory-related duties assigned as required for research projects.
Non-Essential Tasks: Not applicable
Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; B.A. or B.S. degree in Biology, Neuroscience or other appropriate discipline strongly preferred; or equivalent combination of education and experience.
Strong computer skills, including experience with scientific software, Microsoft Office, Photoshop, Illustrator, Endnote, Filemaker, and IMOD
Ability to work in a lab team environment with a diverse group of personnel is essential.
Must be able to work with minimal supervision once trained and to listen to, convey, and exchange information with other laboratory personnel.
This position requires the use of eyes, hands, and fingers with skill and the ability to perform mathematical calculations.
The ability to communicate effectively, both written and verbal; follow instructions, and record information accurately.
Must have excellent interpersonal skills and strong attention to detail.
Ability to work in an independent manner, maintain a safe environment, and exchange information and interact regularly with other laboratory personnel.
The position may entail prolonged periods of standing or sitting.
License/Certification Required institutional certification for laboratory personnel (e.g., laboratory safety training, vivarium, etc.)