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University of Rochester Laboratory Technician II (S) - 229057 in Rochester, New York

Laboratory Technician II (S) Job ID 229057

Location School of Medicine & Dentistry Full/Part Time Full-Time Favorite Job Regular/Temporary Regular Opening

Full Time 40 hours Grade 075 General Neurology Unit


8 AM-4:30 PM



The Department of Neurology is seeking a Full-Time Lab Technician to work in a basic science laboratory and conduct research relating to the vascular complications in HIV infected individuals. The work involves handling HIV infected blood samples.




Under the direct supervision of the Principal Investigator.


Standard office equipment such as multi-feature ACD or smart telephone, PC, copier, printer, scanner, fax machine, and the like. Uses a variety of basic and advanced business/technical programs/applications. Includes systems such as MatLab, Offline Sorter, Microsoft Office, or internally developed databases/systems.


This individual will process blood samples (HIV infected and uninfected) on a daily basis using the protocols already set up in the lab and keep the paperwork associated with it up to date. Initially, the candidate will conduct assigned experiments, working with daily supervision. It is expected that the individual will develop more independence over time in performing the experiments. It is very necessary to strictly adhere to the protocols to avoid manual error in sample processing.

  • Collect blood samples, isolate Peripheral Blood Mononuclear Cells (PBMCs) via density gradient centrifugation using Ficoll reagent, cryopreserve PBMCs. Maintain lab safety and cleanliness. Performs routine maintenance on equipment. (25%)

  • Fix blood samples using 4% Paraformaldehyde and process for cell staining using intracellular as well as cell surface markers of inflammation. Acquire the stained samples using flow cytometry. (20%)

  • Maintain the sample processing and storage log and analyze the flow cytometry data. (20%)

  • Run enzyme-linked immunosorbent assays ( ELISA) for various plasma proteins. (20%)

  • Transfer samples to the central storage facility and attend monthly project related meetings. Perform general laboratory duties such as preparing reagents and restocking supplies. (10%)

  • Other job duties as assigned. (5%)


  • Associates degree in a related field; bachelor’s degree preferred. Previous instruction in theoretical aspects of cell and molecular biology is highly desirable.

  • Strong attention to detail, organizational skills, and experience with basic Cell Culture techniques, DNA extraction and PCR.

  • Excellent interpersonal and communication skills, including written communication.

  • Previous experience with Flow Cytometry, handling blood samples and ELISA is highly desirable.

    How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled