University of Rochester Laboratory Technician III (S) in Rochester, New York
Full Time 40 hours Grade 077 Pharmacology & Physiology
8:30 AM-5 PM
Responsible for the maintenance, breeding, genotyping and organization of multiple transgenic mouse colonies. Additional responsibilities include completing established laboratory functions including cell culture and molecular and cell biological experiments and/or cellular electrophysiology experiments (field recording and/or patch clamp recording). Will assist with overall operations of the laboratory including ordering supplies, making stock solutions, equipment maintenance, and training of lab personnel. Willingness to work with rodents is required. Ability to work in a lab team environment with a diverse group of personnel is essential.
In accordance with broadly-outlined objectives, requirements and approaches, will plan and conduct technical procedures, non-routine and complex in nature. Sets up and operates state-of-the-art equipment or instruments. With latitude for modifying methods and techniques, will analyze data, prepare reports, present data at laboratory meetings, and make recommendations. Will be responsible for ordering laboratory supplies and interacting with vendors. Will organize and manage mouse colonies. Will work with computers, scientific software, Excel, Word, etc.
Breeding of mouse colonies and mouse genotyping. Maintenance and breeding of multiple lines of wild-type transgenic mice. Responsibilities include maintaining breeding records and setting up weekly breeding schedules. Mouse genotyping: Responsibilities include PCR analysis of genomic DNA obtained from ear punches and tail snips, as well as detailed record-keeping of mouse genotypes in laboratory database.
Preparation/maintenance of cell cultures. Generate and maintain cells cultures (e.g. primary cortical neurons, HEK293 cells, etc). Prepare culture media and solutions, feed cells regularly, propagate and freeze cells for later use.
Molecular and cell biological experiments and/or cellular electrophysiology experiments. Work in an independent manner with supervision from the faculty supervisor or other senior laboratory personnel, perform standard molecular biological experiments including cDNA purification, cloning, PCR amplification, restriction analysis, etc. Perform tissue sectioning, immunofluorescence and other cell biological experiments. Optimize assays and modify experiments as needed. May perform cellular electrophysiology recordings (field recordings or patch clamp) to determine the effect of protein phosphatase-1 (PP1) binding proteins on synaptic transmission and plasticity. Working closely with the faculty supervisor. Analyze and to present findings to PI and/or in laboratory meetings. Analyze and to present findings to PI and/or in laboratory meetings.
General laboratory maintenance. Order supplies and work closely with departmental administrative office staff on purchasing and account issues. Prepare common reagents, train other laboratory workers, graduate students, and postdoctoral fellows in relevant laboratory techniques and equipment use. Provide support and technical assistance on experimental problems that may arise during the course of their experiments. Maintain functionality of lab equipment, as well as the overall organization of the lab, and comply with all institutional laboratory safety procedures. Maintain careful laboratory notebooks, analyze data on PCs, and design new protocols.
Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. Prefer B.A. or B.S. degree in Neuroscience, Biology, Chemistry or other appropriate science discipline. We are seeking highly motivated, highly organized graduate to work in a lab team environment with a diverse group of personnel. Fine motor skills is needed. Able to work with minimal supervision once trained and to listen to, convey, and exchange information with other laboratory personnel. This position requires the use of eyes, hands, and fingers with skill and the ability to perform mathematical calculations using algebra and practically apply percentages, fractions, and ratios. The ability to speak and understand English language, follow instructions, and record information accurately is required. Must have excellent interpersonal skills. This position may entail prolonged periods of standing and walking and requires the safe use of toxic chemicals.
EOE Minorities/Females/Protected Veterans/Disabled
Job Title: Laboratory Technician III (S)
Location: School of Medicine & Dentistry
Job ID: 201506
Full/Part Time: Full-Time