University of Rochester Laboratory Technician III (S) in Rochester, New York


Full Time 40 hours Grade 077 Biochemistry and Biophysics





  • Performing molecular biological techniques: plasmid vector construction, analysis and production. Will be shown initially but subsequently techniques will be performed independently.
  • Analysis of molecular biological manipulations: RNA and protein purification from bacterial, insect and mammalian cells, UV/Vis spectrophotometry, agarose gel electrophoresis; PAGE; reverse-transcription-polymerase chain reaction (RT-PCR) and primer design using available computer programs; western blot hybridization; use of Phosphorimaginer to quantitate data. Will be shown initially but subsequently manipulations will be performed independently.Will need to understand theory, think independently, maintain a research notebook (see below), and interpret results.
  • Managing the lab:Maintaining lab equipment including organizing warranty information; placing routine orders for supplies; placing phone calls for service repairs; performing lab surveys for radiation safety; documenting waste/disposal; overseeing all paperwork that pertains to keeping the lab active, making sure the lab is in compliance with all rules and regulations; sending reagents to colleagues who request them and keeping track of what was sent where; and over-seeing the lab aide and his/her functions in preparing clean and RNase-free labware, filing journals, making bacterial plates and simple solutions and running errands. Will be shown initially but subsequently manipulations will be performed independently.Successful candidate will need to self-initiated lab oversight when such oversight is required.
  • Documenting experiments: Requires documentation of all lab activities, procedures and modifications, and experimental results obtained from work done at the bench.This includes not only the personal notebook, but lab notebooks that have information of interest to all lab members.These notebooks document nucleic acids or proteins, the construction of new expression vectors, or the construction of bacterial and mammalian-cell stocks.Responsibility also extends to other lab members, making sure that they properly document all new materials received in the lab.
  • Orienting and training new lab members:Any new graduate students, rotating or permanent, as well as new post-docs and visiting scientists require training in lab methods and procedures. Overseeing undergraduate student lab helper.


Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. Prefer Bachelor degree in biochemistry. Molecular biology, chemistry, or equivalent education and some lab experience (course-work lab is adequate). Must be able to trouble shoot, think independently, know when to seek advice from others when there is a problem, read the literature for technical details, and take initiative. Must also have organizational skills required to manage the operations of a very active research group.

EOE Minorities/Females/Protected Veterans/Disabled

Job Title: Laboratory Technician III (S)

Location: School of Medicine & Dentistry

Job ID: 201890

Regular/Temporary: Regular

Full/Part Time: Full-Time